diff --git a/src/Core/AdminConsole/OrganizationFeatures/OrganizationUsers/AcceptOrgUserCommand.cs b/src/Core/AdminConsole/OrganizationFeatures/OrganizationUsers/AcceptOrgUserCommand.cs
index 756bd2ae46..8c9edb4dc4 100644
--- a/src/Core/AdminConsole/OrganizationFeatures/OrganizationUsers/AcceptOrgUserCommand.cs
+++ b/src/Core/AdminConsole/OrganizationFeatures/OrganizationUsers/AcceptOrgUserCommand.cs
@@ -1,4 +1,5 @@
 using Bit.Core.AdminConsole.Enums;
+using Bit.Core.AdminConsole.OrganizationFeatures.Policies;
 using Bit.Core.AdminConsole.Services;
 using Bit.Core.Auth.Models.Business.Tokenables;
 using Bit.Core.Billing.Enums;
@@ -25,6 +26,8 @@ public class AcceptOrgUserCommand : IAcceptOrgUserCommand
     private readonly IMailService _mailService;
     private readonly IUserRepository _userRepository;
     private readonly IDataProtectorTokenFactory<OrgUserInviteTokenable> _orgUserInviteTokenDataFactory;
+    private readonly IFeatureService _featureService;
+    private readonly IPolicyRequirementQuery _policyRequirementQuery;
 
     public AcceptOrgUserCommand(
         IDataProtectionProvider dataProtectionProvider,
@@ -34,9 +37,10 @@ public class AcceptOrgUserCommand : IAcceptOrgUserCommand
         IPolicyService policyService,
         IMailService mailService,
         IUserRepository userRepository,
-        IDataProtectorTokenFactory<OrgUserInviteTokenable> orgUserInviteTokenDataFactory)
+        IDataProtectorTokenFactory<OrgUserInviteTokenable> orgUserInviteTokenDataFactory,
+        IFeatureService featureService,
+        IPolicyRequirementQuery policyRequirementQuery)
     {
-
         // TODO: remove data protector when old token validation removed
         _dataProtector = dataProtectionProvider.CreateProtector(OrgUserInviteTokenable.DataProtectorPurpose);
         _globalSettings = globalSettings;
@@ -46,6 +50,8 @@ public class AcceptOrgUserCommand : IAcceptOrgUserCommand
         _mailService = mailService;
         _userRepository = userRepository;
         _orgUserInviteTokenDataFactory = orgUserInviteTokenDataFactory;
+        _featureService = featureService;
+        _policyRequirementQuery = policyRequirementQuery;
     }
 
     public async Task<OrganizationUser> AcceptOrgUserByEmailTokenAsync(Guid organizationUserId, User user, string emailToken,
@@ -194,12 +200,7 @@ public class AcceptOrgUserCommand : IAcceptOrgUserCommand
         // Enforce Two Factor Authentication Policy of organization user is trying to join
         if (!await userService.TwoFactorIsEnabledAsync(user))
         {
-            var invitedTwoFactorPolicies = await _policyService.GetPoliciesApplicableToUserAsync(user.Id,
-                PolicyType.TwoFactorAuthentication, OrganizationUserStatusType.Invited);
-            if (invitedTwoFactorPolicies.Any(p => p.OrganizationId == orgUser.OrganizationId))
-            {
-                throw new BadRequestException("You cannot join this organization until you enable two-step login on your user account.");
-            }
+            await ValidateTwoFactorAuthenticationPolicy(user, orgUser.OrganizationId);
         }
 
         orgUser.Status = OrganizationUserStatusType.Accepted;
@@ -220,4 +221,24 @@ public class AcceptOrgUserCommand : IAcceptOrgUserCommand
         return orgUser;
     }
 
+    private async Task ValidateTwoFactorAuthenticationPolicy(User user, Guid organizationId)
+    {
+        if (_featureService.IsEnabled(FeatureFlagKeys.PolicyRequirements))
+        {
+            var requireTwoFactorPolicyRequirement = await _policyRequirementQuery.GetAsync<RequireTwoFactorPolicyRequirement>(user.Id);
+            if (requireTwoFactorPolicyRequirement.RequireTwoFactor)
+            {
+                throw new BadRequestException("You cannot join this organization until you enable two-step login on your user account.");
+            }
+        }
+        else
+        {
+            var invitedTwoFactorPolicies = await _policyService.GetPoliciesApplicableToUserAsync(user.Id,
+                PolicyType.TwoFactorAuthentication, OrganizationUserStatusType.Invited);
+            if (invitedTwoFactorPolicies.Any(p => p.OrganizationId == organizationId))
+            {
+                throw new BadRequestException("You cannot join this organization until you enable two-step login on your user account.");
+            }
+        }
+    }
 }
diff --git a/test/Core.Test/AdminConsole/OrganizationFeatures/OrganizationUsers/AcceptOrgUserCommandTests.cs b/test/Core.Test/AdminConsole/OrganizationFeatures/OrganizationUsers/AcceptOrgUserCommandTests.cs
index 2dda23481a..ae7e6ff094 100644
--- a/test/Core.Test/AdminConsole/OrganizationFeatures/OrganizationUsers/AcceptOrgUserCommandTests.cs
+++ b/test/Core.Test/AdminConsole/OrganizationFeatures/OrganizationUsers/AcceptOrgUserCommandTests.cs
@@ -1,5 +1,6 @@
 using Bit.Core.AdminConsole.Entities;
 using Bit.Core.AdminConsole.Enums;
+using Bit.Core.AdminConsole.OrganizationFeatures.Policies;
 using Bit.Core.AdminConsole.Services;
 using Bit.Core.Auth.Models.Business.Tokenables;
 using Bit.Core.Billing.Enums;
@@ -183,6 +184,96 @@ public class AcceptOrgUserCommandTests
             exception.Message);
     }
 
+    [Theory]
+    [BitAutoData]
+    public async Task AcceptOrgUserAsync_WithPolicyRequirementsEnabled_UserWithout2FAJoining2FARequiredOrg_ThrowsBadRequest(
+        SutProvider<AcceptOrgUserCommand> sutProvider,
+        User user, Organization org, OrganizationUser orgUser, OrganizationUserUserDetails adminUserDetails)
+    {
+        // Arrange
+        SetupCommonAcceptOrgUserMocks(sutProvider, user, org, orgUser, adminUserDetails);
+
+        sutProvider.GetDependency<IFeatureService>()
+            .IsEnabled(FeatureFlagKeys.PolicyRequirements)
+            .Returns(true);
+
+        // User doesn't have 2FA enabled
+        _userService.TwoFactorIsEnabledAsync(user).Returns(false);
+
+        // Organization they are trying to join requires 2FA
+        sutProvider.GetDependency<IPolicyRequirementQuery>()
+            .GetAsync<RequireTwoFactorPolicyRequirement>(user.Id)
+            .Returns(new RequireTwoFactorPolicyRequirement { RequireTwoFactor = true });
+
+        // Act & Assert
+        var exception = await Assert.ThrowsAsync<BadRequestException>(() =>
+            sutProvider.Sut.AcceptOrgUserAsync(orgUser, user, _userService));
+
+        Assert.Equal("You cannot join this organization until you enable two-step login on your user account.",
+            exception.Message);
+    }
+
+    [Theory]
+    [BitAutoData]
+    public async Task AcceptOrgUserAsync_WithPolicyRequirementsEnabled_UserWith2FAJoining2FARequiredOrg_Succeeds(
+        SutProvider<AcceptOrgUserCommand> sutProvider,
+        User user, Organization org, OrganizationUser orgUser, OrganizationUserUserDetails adminUserDetails)
+    {
+        SetupCommonAcceptOrgUserMocks(sutProvider, user, org, orgUser, adminUserDetails);
+
+        sutProvider.GetDependency<IFeatureService>()
+            .IsEnabled(FeatureFlagKeys.PolicyRequirements)
+            .Returns(true);
+
+        // User has 2FA enabled
+        _userService.TwoFactorIsEnabledAsync(user).Returns(true);
+
+        // Organization they are trying to join requires 2FA
+        sutProvider.GetDependency<IPolicyRequirementQuery>()
+            .GetAsync<RequireTwoFactorPolicyRequirement>(user.Id)
+            .Returns(new RequireTwoFactorPolicyRequirement { RequireTwoFactor = true });
+
+        await sutProvider.Sut.AcceptOrgUserAsync(orgUser, user, _userService);
+
+        await sutProvider.GetDependency<IPolicyRequirementQuery>()
+            .DidNotReceiveWithAnyArgs()
+            .GetAsync<RequireTwoFactorPolicyRequirement>(default);
+
+        await sutProvider.GetDependency<IOrganizationUserRepository>()
+            .Received(1)
+            .ReplaceAsync(Arg.Is<OrganizationUser>(ou => ou.Status == OrganizationUserStatusType.Accepted));
+    }
+
+    [Theory]
+    [BitAutoData(true)]
+    [BitAutoData(false)]
+    public async Task AcceptOrgUserAsync_WithPolicyRequirementsEnabled_UserJoiningOrgWithout2FARequirement_Succeeds(
+        bool userTwoFactorEnabled, SutProvider<AcceptOrgUserCommand> sutProvider,
+        User user, Organization org, OrganizationUser orgUser, OrganizationUserUserDetails adminUserDetails)
+    {
+        // Arrange
+        SetupCommonAcceptOrgUserMocks(sutProvider, user, org, orgUser, adminUserDetails);
+
+        sutProvider.GetDependency<IFeatureService>()
+            .IsEnabled(FeatureFlagKeys.PolicyRequirements)
+            .Returns(true);
+
+        // User doesn't have 2FA enabled
+        _userService.TwoFactorIsEnabledAsync(user).Returns(userTwoFactorEnabled);
+
+        // Organization they are trying to join doesn't require 2FA
+        sutProvider.GetDependency<IPolicyRequirementQuery>()
+            .GetAsync<RequireTwoFactorPolicyRequirement>(user.Id)
+            .Returns(new RequireTwoFactorPolicyRequirement { RequireTwoFactor = false });
+
+        // Act
+        await sutProvider.Sut.AcceptOrgUserAsync(orgUser, user, _userService);
+
+        // Assert
+        await sutProvider.GetDependency<IOrganizationUserRepository>().Received(1)
+            .ReplaceAsync(Arg.Is<OrganizationUser>(ou => ou.Status == OrganizationUserStatusType.Accepted));
+    }
+
     [Theory]
     [BitAutoData(OrganizationUserType.Admin)]
     [BitAutoData(OrganizationUserType.Owner)]